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The quantitative measurements presented in the preceding communication (1) made with potassium citrate as a precipitant at pH 6.8 and 0°C. on undiluted serum, differ greatly from the usual results found elsewhere in the literature. The following experiments are now adduced to show that these differences arise from the circumstance that in most of the previous work not all of the essential variables, namely, the pH, temperature, kind of salt, and protein concentration, were controlled in the same manner. As a standard, curves made at pH 6.8 and 0°C. with composite samples of blood serum from 30 or more male rats 100 days old were used, since different series were found to be reproducible and coincident. Individual biological differences had thus been eliminated. Any deviation from this standard could then be demonstrated. In two cases a standard of females was substituted. In these solubility curves several fractions, A, B, C (Ca and C~), and D may be distinguished (1). The effect of ch
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The quantitative measurements presented in the preceding communication (1) made with potassium citrate as a precipitant at pH 6.8 and 0°C. on undiluted serum, differ greatly from the usual results found elsewhere in the literature. The following experiments are now adduced to show that these differences arise from the circumstance that in most of the previous work not all of the essential variables, namely, the pH, temperature, kind of salt, and protein concentration, were controlled in the same manner. As a standard, curves made at pH 6.8 and 0°C. with composite samples of blood serum from 30 or more male rats 100 days old were used, since different series were found to be reproducible and coincident. Individual biological differences had thus been eliminated. Any deviation from this standard could then be demonstrated. In two cases a standard of females was substituted. In these solubility curves several fractions, A, B, C (Ca and C~), and D may be distinguished (1). The effect of ch
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