Response to loading in ex vivo bovine bone
TL;DRAbstract
Aims: The response of bone in vivo towards mechanical loading is widely reported1. However, mechanotransduction studies performed in vitro cannot measure biomechanical parameters in real-time or emulate the 3D structure of bone matrix. The Zetos™ system2 is able to overcome the above limitations. Prior studies have investigated the effects of loading ex vivo bone with intact marrow3-5. The aim of this study was to investigate the effect of mechanical loading on ex vivo trabecular bone without marrow. Methods: Trabecular bone cores (10 x 5 mm) were prepared from a fresh 9-month old steer sternum. Care was taken to maintain sterility and viability at all times. Half of 24 bone cores had their marrow removed. Using custom-made bone culture chambers, they were perfused with culture media (7ml/hr) at 37°C. Three treatment groups of eight samples (four with marrow and four without) were either unloaded or mechanically loaded (2,000 μstrain, 1 Hz, 300 cycles daily or 2,000 μstrain, 1 Hz, 100
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Aims: The response of bone in vivo towards mechanical loading is widely reported1. However, mechanotransduction studies performed in vitro cannot measure biomechanical parameters in real-time or emulate the 3D structure of bone matrix. The Zetos™ system2 is able to overcome the above limitations. Prior studies have investigated the effects of loading ex vivo bone with intact marrow3-5. The aim of this study was to investigate the effect of mechanical loading on ex vivo trabecular bone without marrow. Methods: Trabecular bone cores (10 x 5 mm) were prepared from a fresh 9-month old steer sternum. Care was taken to maintain sterility and viability at all times. Half of 24 bone cores had their marrow removed. Using custom-made bone culture chambers, they were perfused with culture media (7ml/hr) at 37°C. Three treatment groups of eight samples (four with marrow and four without) were either unloaded or mechanically loaded (2,000 μstrain, 1 Hz, 300 cycles daily or 2,000 μstrain, 1 Hz, 100
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