Towards paper-based point-of-care diagnostics fabricated by Laser Induced Forward Transfer

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Towards paper-based point-of-care diagnostics fabricated by Laser Induced Forward Transfer

Ioannis Katis,J. Holloway,Jeppe Madsen,Sebastian Faust,Spiros D. Garbis,Peter J. Smith+4 more-2014-01-01-ePrints Soton (University of Southampton)

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TL;DRAbstract

We report the Laser Induced Forward Transfer (LIFT) of antibodies from a liquid donor film onto paper receivers for application as point-of-care (POC) diagnostic sensors. Paper was chosen as the ideal receiver due to its inherent biocompatibility, wicking properties, wide availability and price, all of which make it an efficient and suitable platform for POC diagnostic sensors. A modified LIFT procedure, referred to as Dynamic Release Layer (DRL-LIFT), has been employed to ensure the viability of the biomolecules post-transfer. The laser used was a KrF excimer operating at 248 nm with a repetition rate of 1 Hz, pulse duration of ~10 ns, and delivering a maximum energy of ~400 mJ per pulse. Both enzyme-tagged and untagged IgG antibodies were LIFT-printed. The functionality and immunological reactivity of the LIFT-printed antibodies was confirmed by developing and demonstrating an Enzyme Linked Immunosorbent Assay and establishing the standard calibration curve for the LIFT-printed pixel

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We report the Laser Induced Forward Transfer (LIFT) of antibodies from a liquid donor film onto paper receivers for application as point-of-care (POC) diagnostic sensors. Paper was chosen as the ideal receiver due to its inherent biocompatibility, wicking properties, wide availability and price, all of which make it an efficient and suitable platform for POC diagnostic sensors. A modified LIFT procedure, referred to as Dynamic Release Layer (DRL-LIFT), has been employed to ensure the viability of the biomolecules post-transfer. The laser used was a KrF excimer operating at 248 nm with a repetition rate of 1 Hz, pulse duration of ~10 ns, and delivering a maximum energy of ~400 mJ per pulse. Both enzyme-tagged and untagged IgG antibodies were LIFT-printed. The functionality and immunological reactivity of the LIFT-printed antibodies was confirmed by developing and demonstrating an Enzyme Linked Immunosorbent Assay and establishing the standard calibration curve for the LIFT-printed pixel

Keywords

LaserLift (data mining)Materials scienceCalibration curveBiomedical engineeringOpticsOptoelectronicsComputer science

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