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Article10.1096/fasebj.21.6.a1325-a

The Role of Cl‐ in the basolateral Na+/Mg2+ exchanger using a TPP+ Electrode

Christie Cefaratti-2007-01-01-The FASEB Journal
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TL;DRAbstract

We have previously demonstrated that the primary Na+/Mg2+ exchanger in hepatocytes is located on the basolateral membrane. This, as well as the fact that basolateral LPM have no Ca2+/Mg2+ exchanger, are the rationale for all membrane transport data performed with basolateral liver plasma membranes (bLPM). The data shown demonstrates that the time course of accumulation and release of TPP+ by basolateral LPM vesicles loaded with MgCl2 was kinetically measured using a TPP+ electrode. Upon the addition of NaCl there was a time dependent decrease in concentration of extravesicular TPP+ indicating an uptake by the vesicles. The release of TPP+ after it reaches steady state levels by the addition of Na+ is likely accounted for by the fact that there are more charge equivalents transported by Mg2+ efflux than carried by Na+ influx, the deficit being compensated by TPP+ accumulation. The time course of TPP+ electrode measurements but in the absence of Cl‐. Under these conditions, a TPP+ accumu

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We have previously demonstrated that the primary Na+/Mg2+ exchanger in hepatocytes is located on the basolateral membrane. This, as well as the fact that basolateral LPM have no Ca2+/Mg2+ exchanger, are the rationale for all membrane transport data performed with basolateral liver plasma membranes (bLPM). The data shown demonstrates that the time course of accumulation and release of TPP+ by basolateral LPM vesicles loaded with MgCl2 was kinetically measured using a TPP+ electrode. Upon the addition of NaCl there was a time dependent decrease in concentration of extravesicular TPP+ indicating an uptake by the vesicles. The release of TPP+ after it reaches steady state levels by the addition of Na+ is likely accounted for by the fact that there are more charge equivalents transported by Mg2+ efflux than carried by Na+ influx, the deficit being compensated by TPP+ accumulation. The time course of TPP+ electrode measurements but in the absence of Cl‐. Under these conditions, a TPP+ accumu

Keywords

ChemistryVesicleEpithelial polarityMembraneIon transporterBiophysicsInorganic chemistryAnalytical Chemistry (journal)

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