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Open AccessArticle10.1590/1808-1657v68n1p0472001

CHEMICAL CONSTITUENTS FROM VITIS VINIFERA (VITACEAE)

J.D. Felício,Raifanny da Silva Oliveira dos Santos,E. Gonçalez-2001-01-01-Arquivos do Instituto Biológico
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TL;DRAbstract

ABSTRACT Optimal conditions for the simultaneous quantitative separation of resveratrol, ɛ - viniferins, balanocarpol, and balanocarpol glycoside a new substance, isolated of Vitis vinifera, were investigated in extracts from leaves and branches. The best results were obtained by the use of reversed - phase high performance liquid chromatography (HPLC) using a mixture of 5% acetic acid and methanol (65:35) as the mobile phase, detector wavelength of 290nm and flow rate of 1mL/ min. The quantification showed the presence of all these compounds in the ethanolic extract from branches, but only the resveratrol was quantified in the ethanolic extracts from leaves.

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ABSTRACT Optimal conditions for the simultaneous quantitative separation of resveratrol, ɛ - viniferins, balanocarpol, and balanocarpol glycoside a new substance, isolated of Vitis vinifera, were investigated in extracts from leaves and branches. The best results were obtained by the use of reversed - phase high performance liquid chromatography (HPLC) using a mixture of 5% acetic acid and methanol (65:35) as the mobile phase, detector wavelength of 290nm and flow rate of 1mL/ min. The quantification showed the presence of all these compounds in the ethanolic extract from branches, but only the resveratrol was quantified in the ethanolic extracts from leaves.

Keywords

VitaceaeVitis viniferaChemical constituentsChemistryBotanyBiologyChromatography

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