Mixed-mode electrokinetic chromatography of low molecular weight anions and cations

TL;DRAbstract

This work presents a comprehensive study into selectivity control over electrokinetic chromatography (EKC) systems for the determination of small organic anions and cations using various additives. For the separation of anions an electrolyte system comprising a cationic soluble polymer (poly( diallydimethylammonium chloride), PDDAC) and a neutral ‚àövº-cyclodextrin (‚àövº-CD) as pseudo-stationary phases was used. The separation mechanism was a combination of electrophoresis, ion-exchange (IE) interactions with PDDAC, and hydrophobic interactions with ‚àövº-CD. The extent of each chromatographic interaction was independently variable, allowing for control of the separation selectivity of the system. Various cationic analytes were also examined, including opiate alkaloids, aromatic bases and amino acids. In the case of the opiate alkaloids (morphine, thebaine, 10-hydroxy thebaine, codeine, oripavine and laudanine) a system utilising sulfated-‚àövº-cyclodextrin (s-‚àövº-C:P) as a pseudo-s

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