User Settings
Open AccessDissertation

Analysis of the Kinesin-13 gene family in plants

Nicholas O. Fischer-2012-01-01-Durham e-Theses (Durham University)
0

TL;DRAbstract

Although the depolymerizing effects of animal kinesin-13 proteins have been well characterized, little is known of the two Arabidopsis kinesin-13 proteins. This project aimed to investigate in more detail the functions of AtKinesin-13A/B and their effect on microtubule dynamics. The first aim was to analyse the function of both proteins in vitro using biochemical studies. Proteins were expressed in both bacterial and insect cells before undergoing co-sedimentation and turbidimetric assays to assess their activity and microtubule-interacting properties. The second aim of this project was to investigate the in vivo effects of these proteins on microtubule dynamics throughout the cell cycle. Tobacco BY2 cell lines were transformed with AtKinesin-13A and 13B, as well as human HsKinesin-13 for comparison under the expression of an estradiol-inducible promoter. This inducible system allowed transient expression of recombinant protein to analyse the effect of expression on cell growth, nuclea

Chat with Paper

AI Agents for this Paper

Although the depolymerizing effects of animal kinesin-13 proteins have been well characterized, little is known of the two Arabidopsis kinesin-13 proteins. This project aimed to investigate in more detail the functions of AtKinesin-13A/B and their effect on microtubule dynamics. The first aim was to analyse the function of both proteins in vitro using biochemical studies. Proteins were expressed in both bacterial and insect cells before undergoing co-sedimentation and turbidimetric assays to assess their activity and microtubule-interacting properties. The second aim of this project was to investigate the in vivo effects of these proteins on microtubule dynamics throughout the cell cycle. Tobacco BY2 cell lines were transformed with AtKinesin-13A and 13B, as well as human HsKinesin-13 for comparison under the expression of an estradiol-inducible promoter. This inducible system allowed transient expression of recombinant protein to analyse the effect of expression on cell growth, nuclea

Keywords

MicrotubuleKinesinCell biologyMetaphaseBiologyCell cycleMicrotubule-associated proteinTubulin

Chat

Click to start Chat