Capacity of leaf extracts of tobacco to react with ozone

TL;DRAbstract

The capacity of leaf extracts of tobacco to react with ozone was tested by measuring the ability of these extracts to inhibit the peroxidation of methyl linolenate exposed to ozone. Methyl linolenate (60 g) suspended in 3 ml neutral 0.025 M phosphate buffer plus 40 g of sodium lauryl sulfate and 10% ethanol gave a linear increase in peroxidation when ozonated for 1-4 min by an air stream containing 6 ppm ozone bubbled through at the rate of 1 liter/min. Peroxidation was measured as the increase in malonyl dialdehyde (MDA). Cysteine (20, 40, and 60 g), added before ozonation, inhibited MDA formation 45, 55, and 65%, respectively, when ozonated for 2 min, and 10, 30, and 45% when ozonated for 4 min. Juice expressed from a single frozen disc (3 cm-diameter) of tobacco leaf (Nicotiana tabacum) also inhibited formation of MDA when added before ozonation. After 2 min of ozonation, juice from an ozone-resistant and an ozone-susceptible cultivar inhibited the formation of MDA 52 and 55%, respe

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