Identification of differential HLA-binding peptide between two hepatoma cell lines HepG2 and HepG2.2.15

TL;DRAbstract

AIM: To screen the differential HLA-binding peptides between HepG2 and HepG2.2.15 cell lines, and to find some HLA-binding peptides correlated with hepatitis B virus (HBV) infection. METHODS: HepG2 and HepG2.2.15 cells were harvested (10^8 cells), and the peptides were isolated from the cell membrane by mild acid elution, respectively. Then the mixture of peptides was fractionated by high performance liquid chromatography (HPLC) and the differential fractions only expressed in HepG2.2.15 cell line were identified by nanoESI-MS/MS analysis. Bioinformatic analysis and MASCOT index were used to investigate the sequence and source of the peptides. Finally the expression of mRNA was detected by reverse transcription-polymerase chain reaction (RT-PCR). RESULTS: HPLC fractions were markedly different between HepG2 and HepG2.2.15 cells. A peptide, SPDDPSRYISPDQ, from enolase 1 (ENO1) was obtained, which was only expressed in HepG2.2.15 cells, by nanoESI-MS/MS analysis. The result of RT-PCR con

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