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Background: Many studies have demonstrated that 5-aminosalicylic acid (5-ASA) and non-steroidal anti- inflammatory drugs (NSAIDs) have chemoprevention effect on colorectal cancer. However, few studies have been published to date referring to the effect of coadministration of the two drugs. Aims: To investigate the inhibitory effect of 5-ASA in combination with nimesulide (a selective cyclooxygenase-2 inhibitor) on the proliferation of human colonic cancer cell line HT-29 and its potential mechanisms. Methods: The inhibitory effect of 5-ASA and/or nimesulide on HT-29 cells was determined by methyl thiazolyl tetrazolium (MTT) assay. Apoptosis and expression of proliferating cell nuclear antigen (PCNA) were detected by TdT-mediated dUTP-biotin nick-end labeling (TUNEL) assay and immunocytochemical staining, respectively. Results: After treatment with 5-ASA or nimesulide alone at the concentrations of 1 to 1000µmol/L, the proliferation of HT-29 cells in vitro was inhibited in a dose-depend
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Background: Many studies have demonstrated that 5-aminosalicylic acid (5-ASA) and non-steroidal anti- inflammatory drugs (NSAIDs) have chemoprevention effect on colorectal cancer. However, few studies have been published to date referring to the effect of coadministration of the two drugs. Aims: To investigate the inhibitory effect of 5-ASA in combination with nimesulide (a selective cyclooxygenase-2 inhibitor) on the proliferation of human colonic cancer cell line HT-29 and its potential mechanisms. Methods: The inhibitory effect of 5-ASA and/or nimesulide on HT-29 cells was determined by methyl thiazolyl tetrazolium (MTT) assay. Apoptosis and expression of proliferating cell nuclear antigen (PCNA) were detected by TdT-mediated dUTP-biotin nick-end labeling (TUNEL) assay and immunocytochemical staining, respectively. Results: After treatment with 5-ASA or nimesulide alone at the concentrations of 1 to 1000µmol/L, the proliferation of HT-29 cells in vitro was inhibited in a dose-depend
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