TL;DRAbstract
Pseudomonas putida ZWL73, which completely degrades 4-chloronitrobenzene (4-CNB), can grow on the media with 3-hydroxybenzoate (3-HBA) as a sole carbon source and energy source. During the course of the degradation of 3-HBA, gentisate was produced as an intermediate. Through spectrophotometric analyses on cell extracts of ZWL73 induced with 3-HBA, it was indicated that glutathione (GSH) -dependent gentisate pathway is involved in the 3-HBA catabolism in ZWL73 and the catabolism of this pathway must be induced with 3-HBA.
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Pseudomonas putida ZWL73, which completely degrades 4-chloronitrobenzene (4-CNB), can grow on the media with 3-hydroxybenzoate (3-HBA) as a sole carbon source and energy source. During the course of the degradation of 3-HBA, gentisate was produced as an intermediate. Through spectrophotometric analyses on cell extracts of ZWL73 induced with 3-HBA, it was indicated that glutathione (GSH) -dependent gentisate pathway is involved in the 3-HBA catabolism in ZWL73 and the catabolism of this pathway must be induced with 3-HBA.
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