Regulatory effect of compatibility of Astragalus membranaceus and Rhizoma curcumae on COX-2 expression in gastric cancer MKN-45 cells

TL;DRAbstract

AIM: To investigate the effect of Astragalus membranaceus and Rhizoma curcumae's compatibility on MKN-45 cells and the regulatory action of the compatibility on expression of cyclooxygenase 2 (COX-2), peroxisome proliferators activated receptorγ (PPARγ) and nuclear factor κB (NF-κB). METHODS: The compatibility of Astragalusmembranaceus and Rhizoma curcumae was used on MKN-45 cells and there were five groups including Celecoxib group, Rosigli-tazone group, Astragalus membranaceus group, Rhizoma curcumae group and control group. The inhibition ratio in each group was deter-mined using MTT method, and the expressions of COX-2 mRNA, PPARγ mRNA, NF-κB mRNA and COX-2 protein were measured using RT-PCR and Western blot methods. RESULTS: Both the Chinese drugs and Western medicines had suppression on NF-κB mRNA and COX-2 mRNA. All medicines except Rhizoma cur-cumae promoted the expression of PPARγ mRNA. The most obvious suppressive effect on COX-2 mRNA expression was detected in celecoxib grou

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