Studies on a novel insertion sequence, ISR11, isolated from rhizobium leguminosarum biovar viciae

TL;DRAbstract

An insertion sequence, ISRll, isolated from Rhizobium
\nleguminosarum biovar viciae was studied in detail. Both
\nstrands of the entire element were sequenced. The inverted
\nrepeats (13bp) and target duplications (8bp) of the element
\nhad features in common with three other IS elements, I S R m 2 , IS66 and IS866 (from Rhizobium meliloti and Agrobacterium
\ntumefaciens).
\n
\nThe GENBANK and PIR sequence databases were searched
\nfor similarities to ISRll. The homologies found were to
\nother insertion sequences, unidentified open reading frames
\nand to several genes. All these sequences were from species
\nof Rhizobiaceae and all mapped to four small regions of the
\nelement.
\n
\nTwo approaches were taken to identifying coding regions
\nwithin ISRll. Gene search by signal identified twenty
\ntwo open reading frames. The sequence of all these ORFs was
\ntranslated to protein sequences and compared to the sequence&#

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