利用PCR-SacII-RFLP技术检测绵羊IGFBP-3基因多态性的研究

TL;DRAbstract

【Objective】 To seek the SNP associated with the economic traits such as wool traits in sheep, IGFBP-3 regarded was as candidate gene reflecting growth and development. 【Method】 The polymorphism of sheep IGFBP-3 gene was first detected by PCR-SSCP and DNA sequencing. On the basis, the PCR-RFLP method for the special SNP was established and used to identify the genotype and allele frequencies in different sheep breeds (native breeds HU sheep, Small-tail Han sheep and Kazakh sheep, breeding variety Chinese Merino sheep, foreign breed Suffolk sheep and Dorper sheep). The association between different genotypes and several wool traits of Chinese Merino sheep was analyzed by multi-comparison method. 【Result】 An expected PCR product of 249 bp was amplified using the sheep genomic DNA as temple. PCR-SSCP and sequence analysis revealed a G/T transversion at position 140 of the fragment, resulting in the SacⅡ site disappeared. Digested by SacⅡ to the PCR product, Kazakh sheep and Chinese Merino

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