&lt;b&gt;Calcitonin secretion in a primary culture of isolated ultimobranchial bodies of rat &lt;/b&gt;&lt;b&gt;fetuses &lt;/b&gt;

doi:https://doi.org/10.2220/biomedres.13.81

Open AccessArticle10.2220/biomedres.13.81

<b>Calcitonin secretion in a primary culture of isolated ultimobranchial bodies of rat </b><b>fetuses </b>

Ichiro Nishiyama,Tomoko Fujii-1992-01-01-Biomedical Research

TL;DRAbstract

Calcitonin (CT) secretion from fetal rat C-cells was studied in a primary culture of ultimobranchial bodies (UBs). The UBs were enzymatically isolated from thyroid-parathyroid-UB complexes of 16-day rat fetuses, and cultured for 96 h. The purity of the C-cells in the cultures was estimated to be about 70% by an immunociytochemical procedure using anti-CT antiserum. CT secretion from the cultured C-cells was significantly stimulated by raised medium Ca (2-5 mM), BAY K 8644 (10 ,uM), raised medium K (50 mM) or dibutyryl cyclic AMP (1 mM). These results suggested that two pathways in the CT secretion mediated by Ca2+ and by cAMP are active in the C-cells of rat fetuses.

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Calcitonin (CT) secretion from fetal rat C-cells was studied in a primary culture of ultimobranchial bodies (UBs). The UBs were enzymatically isolated from thyroid-parathyroid-UB complexes of 16-day rat fetuses, and cultured for 96 h. The purity of the C-cells in the cultures was estimated to be about 70% by an immunociytochemical procedure using anti-CT antiserum. CT secretion from the cultured C-cells was significantly stimulated by raised medium Ca (2-5 mM), BAY K 8644 (10 ,uM), raised medium K (50 mM) or dibutyryl cyclic AMP (1 mM). These results suggested that two pathways in the CT secretion mediated by Ca2+ and by cAMP are active in the C-cells of rat fetuses.

Keywords

Vasoactive intestinal peptideLamina propriaMedicineFetusUlcerative colitisImmunohistochemistrySecretionCalcitonin

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