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HPLC Analysis of nine corticosteroids in “natural creams” for atopic eczema

Agim Ameti,Poposka, Zaklina,Shaban Memeti,Maja Shishovska,Zana Mustafa,Katerina Starkoska+1 more-2013-09-28-Goce Delchev University Repository (Goce Delčev University of Štip)
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Purpose: The aim of the study was to determine whether “natural creams” sold for treatment of childhood atopic eczema illegally contain corticosteroids with a newly developed rapid and simple HPLC analysis with UV detection. Material and Methods: HPLC analysis was performed using a Schimadzu LC-2010 chromatographic system (Schimadzu, Kyoto, Japan) consisting of a LC-20AT Prominence liquid chromatography pump with DGU-20A5 Prominence degasser, a SPD-M20A Prominence Diode Array Detector, and a SIL-20 AC Prominence auto sampler. Data analyses were done using Class VP 7.3 Software. The elution was carried out on a column Purospher STAR® RP 18e (250 x 4.6 mm i.d., particle size 5m), with a mobile phase consisted of acetonitrile and water in a gradient mode, at a flow rate of 1.0 mL min-1, at controlled column temperature (25oC). Detection of nine different corticosteroids (dexamethasone, prednisolone, methylprednisolone, fluocortolone, hydrocortisone, mometasone, betamethasone, beclomethas

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Purpose: The aim of the study was to determine whether “natural creams” sold for treatment of childhood atopic eczema illegally contain corticosteroids with a newly developed rapid and simple HPLC analysis with UV detection. Material and Methods: HPLC analysis was performed using a Schimadzu LC-2010 chromatographic system (Schimadzu, Kyoto, Japan) consisting of a LC-20AT Prominence liquid chromatography pump with DGU-20A5 Prominence degasser, a SPD-M20A Prominence Diode Array Detector, and a SIL-20 AC Prominence auto sampler. Data analyses were done using Class VP 7.3 Software. The elution was carried out on a column Purospher STAR® RP 18e (250 x 4.6 mm i.d., particle size 5m), with a mobile phase consisted of acetonitrile and water in a gradient mode, at a flow rate of 1.0 mL min-1, at controlled column temperature (25oC). Detection of nine different corticosteroids (dexamethasone, prednisolone, methylprednisolone, fluocortolone, hydrocortisone, mometasone, betamethasone, beclomethas

Keywords

ChromatographyBetamethasoneDetection limitHigh-performance liquid chromatographyChemistryMedicineInternal medicine

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