Development of Adenoviral Vectors for Studying Hematopoietic Stem Cell Function

TL;DRAbstract

Hematopoietic stem cells (HSCs) are defined by their capacity to self-renew and differentiate into all hematopoietic cell lineages. Postnatally, HSCs reside predominantly in the bone marrow and are relatively rare, but important cells because of their therapeutic potential in bone marrow transplantation protocols and in their involvement in the origin of certain leukemias. To study how various genetic programs govern the regulation of HSCs, replication deficient viral vectors, including adenoviral vectors, have been utilized for gene transfer and expression in normal and malignant hematopoietic progenitor and stem cells. Adenoviral vectors allow high levels of transient transgene expression, however, adenoviral transduction of hematopoietic cells has been hampered by low expression of the coaxackie and adenovirus receptor (CAR), which is the primary target cell receptor for adenovirus serotype 5 (Ad5). With the aim to increase adenoviral gene transfer into hematopoietic cells, we modif

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