IL-10对脂多糖诱导Ana-1细胞的MyD88/核因子κB活性影响的研究

TL;DRAbstract

Objective To investigate the effects of IL-10 on lipopolysaccharide (LPS)-induced MyD88/NF-KB signaling activation. Methods Ana-1 macrophages were divided into a LPS group and a LPS+IL-10 group. The cells and the culture supernatant were collected at 0, 0.5, 1, and 2 hours respectively. The expression levels of NF-KB p65 and MyD88 in cytoplasm and nucleus were detected by Western blotting. The concentration of TNF-α in the culture supernatant was determined by ELISA. Results Through 0 to 2 hours, MyD88 expression increased significantly after LPS stimulation. The expression was attenuated by the pretreatment of IL-10, which returned to normal levels at 2 hours (8.8+0.3 vs 21.4±1.8, P＜0.05). IL-10 had no effect on total expression of NF-kB, but decreased nuclei/cytoplasm ratio of NF-kB p65 after LPS stimulation. The ratio was lower in the LPS+IL-10 group compared and the LPS group at 1 hour and 2 hour (1.1±0.1 vs 2.4±0.4, 0.6±0.7 vs 3.1±0.6, P＜0.05). Consequently, IL-10 pretreatment dec

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