Die Auswirkung von verschiedenen Proteasom-Inhibitoren auf die Wallersche Degeneration peripherer Nerven in vitro und in vivo

TL;DRAbstract

The present study examined the effects of proteasome inhibitors on Wallerian degeneration (WD) in the peripheral nervous system on the example of sciatic nerve sectioning in vivo and on the basis of nerve-macrophage co-cultures in vitro. The Wallerian degeneration describes the processes of discontinuity in the nervous system by chemical-toxic, metabolic or mechanical causes. The ubiquitin-proteasome-system (UPS), in addition to the lysosome, provides most of the proteolysis and is one of the main effectors of coordinating all processes running for protein degradation. It is hoped to be able to influence the degeneration of peripheral nerves by inhibiting this system. To display the events of the WD in vitro, a time series of tissue cultures without inhibitor over a period of eight days is first made. Cultures are compared with and without the addition of peritoneal macrophages. Lactacystin and MG132 are used as proteasome inhibitors for the actual experiments, the latter is used only

Chat with Paper

AI Agents for this Paper