Alterations in mRNA and protein profiles in eIF4E-transfected human lung carcinoma cells

TL;DRAbstract

Previous work has shown that treatment of the lung cell carcinoma cell line DLKP with the differentiation modulating agent bromodeoxyuridine (BrdU) causes posttranscriptionally regulated changes in the expression of growth and differentiation related genes. These changes in gene expression were found to coincide with an increase in the level of expression and phosphorylation of the translation initiation factor eIF-4E. In this study we have overexpressed eIF4E in DLKP cells to determine its role in mediating the changes seen in BrdU treatment and what effects it may have on the growth of lung cancer cells in general as studies have shown eIF4E to play a role in regulating gene expression in carcinogenesis We also analysed the overexpression of Ser209 mutated non-phosphorylatable eIF4E in DLKP cells to determine the role of the eIF4E Ser209 (S209) phosphorylation site in regulating translational changes in gene expression and functional changes in DLKP cells. The exact role of eIF4E Ser

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