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Cytochrome P-450 in drug-resistant Mycobacterium tuberculosis

Geetha Ramachandran,Prema Gurumurthy,Narayanan Parameswaran,Usha Mahadevan-1999-05-01-NOT FOUND REPOSITORY (Indian Institute of Science Bangalore)
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TL;DRAbstract

Methods for the isolation and spectrophotometric determination of cytochrome P-450 in mycobacteria were standardized. Cytochrome P-450 levels were estimated in Mycobacterium tuberculosis organisms sensitive to both isoniazid and rifampicin and resistant to any of the two drugs. Cytochrome P- 450 was isolated and its presence was shown in M. smegmatis, M. fortuitum, M. chelonae and M. tuberculosis H(37)Rv. The cytochrome P-450 content was significantly elevated in M. tuberculosis, resistant to both isoniazid and rifampicin when compared with the corresponding sensitive strains. It therefore appears that cytochrome P-450 might play a role in causing drug resistance in tuberculosis.

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Methods for the isolation and spectrophotometric determination of cytochrome P-450 in mycobacteria were standardized. Cytochrome P-450 levels were estimated in Mycobacterium tuberculosis organisms sensitive to both isoniazid and rifampicin and resistant to any of the two drugs. Cytochrome P- 450 was isolated and its presence was shown in M. smegmatis, M. fortuitum, M. chelonae and M. tuberculosis H(37)Rv. The cytochrome P-450 content was significantly elevated in M. tuberculosis, resistant to both isoniazid and rifampicin when compared with the corresponding sensitive strains. It therefore appears that cytochrome P-450 might play a role in causing drug resistance in tuberculosis.

Keywords

RifampicinIsoniazidMycobacterium tuberculosisCytochromeMicrobiologyTuberculosisMycobacterium smegmatisDrug

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