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Production of Cellulases by the Endophytic Fungus Fusarium oxysporum

Onofre Sideney Becker-2013-08-02-Journal of Microbiology Research
17

TL;DRAbstract

Cellulose has enormous potential as a renewable energy source and the application of cellulases in the conversion of cellulolytic biomass may provide great economical benefits; in addition, cellulases can be used in a wide range of industrial applications.Given the biotechnological importance of cellulases, the aim of this study was to evaluate the production of cellulases by endophytic fungi of the genus <i>Fusarium</i> <i>oxysporum</i> isolated from <i>Baccharis dracunculifolia</i> D.C. (Asteraceae). The studies were conducted using a basic substrate of sugarcane bagasse that was pretreated for complete removal of the sugar content. The material was dried at 28<SUP>°</SUP>C for 96 days and quantified every seven days after 25 days of fermentation. To quantify the enzymes, the indirect spectrophotometric method was used, with DNS reagent. The results showed that the greatest peak of enzyme production was at 55 days of fermentation, with a yield of 55.21 ± 10.54 IU/g of fermented sub

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Cellulose has enormous potential as a renewable energy source and the application of cellulases in the conversion of cellulolytic biomass may provide great economical benefits; in addition, cellulases can be used in a wide range of industrial applications.Given the biotechnological importance of cellulases, the aim of this study was to evaluate the production of cellulases by endophytic fungi of the genus <i>Fusarium</i> <i>oxysporum</i> isolated from <i>Baccharis dracunculifolia</i> D.C. (Asteraceae). The studies were conducted using a basic substrate of sugarcane bagasse that was pretreated for complete removal of the sugar content. The material was dried at 28<SUP>°</SUP>C for 96 days and quantified every seven days after 25 days of fermentation. To quantify the enzymes, the indirect spectrophotometric method was used, with DNS reagent. The results showed that the greatest peak of enzyme production was at 55 days of fermentation, with a yield of 55.21 ± 10.54 IU/g of fermented sub

Keywords

CellulaseFusarium oxysporumBagasseFermentationCelluloseFood scienceBiomass (ecology)Botany

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