Biology of inerleukin-7 variant isoforms

TL;DRAbstract

<p>The interleukin-7 (IL-7) gene produces nine alternatively spliced mRNA variants. Until now, the clinical and biological significance of alternatively spliced IL-7 products has not been well defined. In this thesis, we investigated IL-7 gene alternative splicing in association with chronic inflammation and infection. This thesis also investigated the biology of alternatively spliced IL- 7 variants in T-cell subsets and CD14+ monocytes. We identified for the first time the alternatively spliced IL-7δ5 variant protein among IL-7 protein variants in healthy and diseased human tissue.</p><p>In paper I, we investigated the extent and impact of IL-7 pre-mRNA alternative splicing in Peripheral Blood Mononuclear Cells (PBMCs) from 16 patients with multiple sclerosis and 15 healthy individuals by transcript specific amplification of interleukin-7 and the interleukin-7 receptor gene. The relative abundance of the gene transcripts was correlated to health and disease (MS) stat

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