Enzymatic production of small interfering RNAs

TL;DRAbstract

RNA interference is a powerful natural phenomenon of post-transcriptional gene silencing that has been found in several biological systems. Small interfering RNAs (siRNAs) are important reagents in the RNA interference pathways that determine gene-specificity of the pathway. RNA interference can be induced in a system by the application of artificial siRNAs. Small interfering RNAs are vital intermediates in natural RNA interference that determine gene specificity of the phenomenon. RNA interference can be engineered for a gene for which this process does not occur in nature by applying artificial siRNAs, to study its function or for therapeutic purpose (Dykxhoorn et al., 2003). The original way of producing siRNAs was by chemical synthesis, which is expensive and usually there is no guarantee that reagents would produce desired gene silencing effects. A number of laboratories have developed alternative methods of producing siRNAs involving plasmid- or virus-mediated intra cellular expr

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