Human amniotic epithelial cells as feeder layer to derive and maintain human embryonic stem cells from poor-quality embryos

doi:https://doi.org/10.1016/j.scr.2015.07.006

Open AccessArticle10.1016/j.scr.2015.07.006

Human amniotic epithelial cells as feeder layer to derive and maintain human embryonic stem cells from poor-quality embryos

Daniela Ávila‐González,Eva Vega-Hernández,Juan Carlos Regalado-Hernández,Julio Francisco De la Jara-Díaz,Irma Lydia García‐Castro,Anayansí Molina‐Hernández+7 more-2015-07-26-Stem Cell Research

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TL;DRAbstract

Data from the literature suggest that human embryonic stem cell (hESC) lines used in research do not genetically represent all human populations. The derivation of hESC through conventional methods involve the destruction of viable human embryos, as well the use of mouse embryonic fibroblasts as a feeder layer, which has several drawbacks. We obtained the hESC line (Amicqui-1) from poor-quality (PQ) embryos derived and maintained on human amniotic epithelial cells (hAEC). This line displays a battery of markers of pluripotency and we demonstrated the capacity of these cells to produce derivates of the three germ layers.

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Data from the literature suggest that human embryonic stem cell (hESC) lines used in research do not genetically represent all human populations. The derivation of hESC through conventional methods involve the destruction of viable human embryos, as well the use of mouse embryonic fibroblasts as a feeder layer, which has several drawbacks. We obtained the hESC line (Amicqui-1) from poor-quality (PQ) embryos derived and maintained on human amniotic epithelial cells (hAEC). This line displays a battery of markers of pluripotency and we demonstrated the capacity of these cells to produce derivates of the three germ layers.

Keywords

BiologyEmbryonic stem cellGerm layerCell biologyAmniotic epithelial cellsEmbryoStem cellKOSR

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