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Article10.4049/jimmunol.184.supp.43.27

Characterization of intestinal distension in ELT-2kd <i>Caenorhabditis elegans</i> upon infection with <i>Pseudomonas aeruginosa</i> (43.27)

Samantha Elliott,Craig Sturgeon,Madeline C. Montgomery-2010-04-01-The Journal of Immunology
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TL;DRAbstract

Abstract Caenorhabditis elegans has become a popular model for studying innate immunity. Recently, the transcription factor ELT-2, which is homologous to human GATA-4, -5 and -6, has been shown to play a role in the nematode immune response, along with its established role in intestinal development. One characteristic of ELT-2’s role in innate immunity is the accumulation of bacteria and distension of the intestinal lumen when ELT-2 is downregulated in the nematode by RNA interference. However, the mechanism of this distension remains unclear. In this study, we characterize the timeline of intestinal distension in ELT-2kd and ELT-7kd nematodes during Pseudomonas aeruginosa infection. We show that intestinal distension is seen in ELT-2kd but not ELT-7kd nematodes during P. aeruginosa infection. Significant distension in ELT-2kd nematodes is seen within 30 hours of pathogen exposure. A detailed comparison of this distension with survival of the nematodes on the pathogen will be presented

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Abstract Caenorhabditis elegans has become a popular model for studying innate immunity. Recently, the transcription factor ELT-2, which is homologous to human GATA-4, -5 and -6, has been shown to play a role in the nematode immune response, along with its established role in intestinal development. One characteristic of ELT-2’s role in innate immunity is the accumulation of bacteria and distension of the intestinal lumen when ELT-2 is downregulated in the nematode by RNA interference. However, the mechanism of this distension remains unclear. In this study, we characterize the timeline of intestinal distension in ELT-2kd and ELT-7kd nematodes during Pseudomonas aeruginosa infection. We show that intestinal distension is seen in ELT-2kd but not ELT-7kd nematodes during P. aeruginosa infection. Significant distension in ELT-2kd nematodes is seen within 30 hours of pathogen exposure. A detailed comparison of this distension with survival of the nematodes on the pathogen will be presented

Keywords

BiologyPseudomonas aeruginosaMicrobiologyInnate immune systemDistensionCaenorhabditis elegansPathogenNematode

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