Selection and genetic improvement of microorganisms for second generation bioethanol.

TL;DRAbstract

Bioethanol produced from biomass is considered an interesting second generation biofuel. To date a cost-effective method for converting biomass into ethanol has not been developed. Consolidated BioProcessing is one of the most attractive strategies aiming to obtain ethanol from biomass by a single microbial phase. In this study, the selection and genetic improvement of microbial strains started in order to develop a microbe for the one-step bioconversion of biomass into ethanol. New efficient cellulolytic microrganisms were isolated and genetically identified. Their hydrolytic activities were remarkable and few strains may have also improvable ethanol production properties. Four hundred wild type yeasts, having optimal fermentative performance, were evaluated for their extracellular enzymatic activities. The yeasts showed interesting hydrolytic activity on pectin, cellulose and starch. In particular, one non-Saccharomyces strain produced efficient cellulolytic enzymes and thirteen S. c

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