Abstract 5669: Monocytic Cells Are Required for Endothelial Regeneration

TL;DRAbstract

Background: Circulating endothelial progenitor cells (EPC) contribute to endothelial cell (EC) regeneration and are predictors for cardiovascular events. Systemic treatment with EPC fosters the repair of damaged endothelium. We have shown that transfusion of mononuclear cells (MNC) lead to an even more pronounced regeneration of the endothelium compared to EPC transfusion. Here, we determine the role of monocytic CD11b+ cells in EC regeneration. Methods/Results Re-endothelialization, neointima formation, and endothelial function was determined in wild-type (WT) mice treated with intravenous injections of spleen-derived MNC, CD11b-depleted MNC, CD11b+ cells, or cell-free vehicle after EC denudation. EC regeneration was enhanced and neointima formation reduced after transfusion of total MNC and CD11b+ cells but not after CD11b-depleted cells. We further determined the role of monocytes in a model of a disseminated EC damage. In ApoE−/− mice, transfusion of CD11b+ cells and total MNC but

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