Combined analysis of microRNA and mRNA signatures in human hematopoietic stem and progenitor cells using a novel microarray quantification system

TL;DRAbstract

MicroRNAs (miRNAs), short non-coding RNAs of ~ 21 to 23 nucleotides in length, regulate target mRNAs post-transcriptionally. They play an important role in many different cellular, developmental, and physiological processes including hematopoiesis. It was the aim of this study to characterize the miRNA expression in human hematopoietic stem and progenitor cells. Hematopoietic stem cells (HSCs) have the ability to generate all different kinds of blood cells and express a number of specific surface markers such as CD34 or CD133. As CD133+ cells appear to be ancestral to CD34+ cells, the miRNA profile of these cells could broaden the knowledge of the miRNA role in the differentiation of CD133+ cells. To analyze the function of miRNAs in HSCs, a miRNA microarray platform (miRXploreTM) was developed. The platform was used, inter alia, to validate sequencing data of Small RNA libraries. Standard array experiments measure relative expression levels. However, absolute expression levels in term

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