Neue Biofilminhibitoren mittels Metagenom-Strategie und marine Streptomyceten, neue Naturstoffe, Synthesen und Biosynthesen
TL;DRAbstract
This thesis is divided in four separate sections. The first part deals with the synthesis and chemical analysis of novel biofilm inhibitors as a part of a joint research project for the identification of novel biofilm inhibitors from metagenomes. The quorum sensing molecules autoinducer AI-1 (AI-1) and autoinducer AI-2 (AI-2) were synthesized by total synthesis and purification after enzymatical preparation for biochemical investigations in sufficient amounts and without toxic by-products. Furthermore the quorum quenching activity of metagenome-derived clones (bpiB genes) which inhibited the formation of biofilms was verified as lactonase activity by HPLC-ESI-MS analysis.The second topic of this thesis is about the Streptomyces sp. strain JP90, isolated as an associated bacterium from marine invertebrates (cooperation with Prof. Dr. J. Piel, Bonn). The secondary metabolite cinnamoylphosphoramide comprises an unusual phosphoramide methyl ester moiety which is rare among natural products
Chat with Paper
AI Agents for this Paper
This thesis is divided in four separate sections. The first part deals with the synthesis and chemical analysis of novel biofilm inhibitors as a part of a joint research project for the identification of novel biofilm inhibitors from metagenomes. The quorum sensing molecules autoinducer AI-1 (AI-1) and autoinducer AI-2 (AI-2) were synthesized by total synthesis and purification after enzymatical preparation for biochemical investigations in sufficient amounts and without toxic by-products. Furthermore the quorum quenching activity of metagenome-derived clones (bpiB genes) which inhibited the formation of biofilms was verified as lactonase activity by HPLC-ESI-MS analysis.The second topic of this thesis is about the Streptomyces sp. strain JP90, isolated as an associated bacterium from marine invertebrates (cooperation with Prof. Dr. J. Piel, Bonn). The secondary metabolite cinnamoylphosphoramide comprises an unusual phosphoramide methyl ester moiety which is rare among natural products
Keywords
Chat
Click to start Chat