Comparative Study of Raman micro spectroscopy and FTIR in Colorectal Tumor Model

TL;DRAbstract

概 要 FTIR spectroscopy and Raman micro-spectroscopy were used to differentiate between grade levels of non clinic samples, colorectal tumor models at 4 different grades; normal, grade 1, grade 2 and grade 3. Detailed investigation of the spectra in the fingerprint region (4000–500 cm-1) revealed some distinct peaks and shoulders, most of which were assignable to wave numbers that shown to represent biochemical changing within the tissue. Differences in peak heights and peak ratios indicated differences in biochemical composition of cancer from different grade level. It was possible to distinguish between their grades. However, all collected colorectal tumor model at different peak was distinguishable using Raman micro-spectroscopy and FTIR clearly. The nucleus information (grouping in PCR) of H-E (hematoxylin-eosin) staining image is very nice correlation with P=O component. Antisym. P=O component (stretch. vib.) has higher correlation with antisym stretch. of CH2 and C=O component at 5

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