Abstract 5130: Leptin Increases Perihematomal Inflammation in Experimental Hemorrhagic Stroke
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Background : Leptin, one of the most alleged adipokines, exerts its tissue-protective effect in ischemic injuries by reducing apoptosis through extracellular signal-regulated kinase pathway. In this context, it may be speculated that leptin also confer beneficial effect on hemorrhagic events. However, few studies have elaborated on the association between exogenous leptin and tissue damage in experimental hemorrhagic stroke model. Methods : After inducing intracerebral hemorrhage in mouse brain via the sterotaxic infusion of collagenase, 4mg/kg or 8mg/kg of recombinant leptin or vehicle (phosphate buffered saline) was administered intraperitoneally. Brain water content, reflecting peri-hematomal edema, and hemorrhage volume were measured at 72 hours after inducing hemorrhage. Inflammatory cells expressing myeloperoxidase (MPO) or OX6 were counted at 48 hours, and western blotting for Akt, pAkt, STAT3, pSTAT3, ERK and pERK was performed at 24 hours. Results : Compared to the vehicle inj
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Background : Leptin, one of the most alleged adipokines, exerts its tissue-protective effect in ischemic injuries by reducing apoptosis through extracellular signal-regulated kinase pathway. In this context, it may be speculated that leptin also confer beneficial effect on hemorrhagic events. However, few studies have elaborated on the association between exogenous leptin and tissue damage in experimental hemorrhagic stroke model. Methods : After inducing intracerebral hemorrhage in mouse brain via the sterotaxic infusion of collagenase, 4mg/kg or 8mg/kg of recombinant leptin or vehicle (phosphate buffered saline) was administered intraperitoneally. Brain water content, reflecting peri-hematomal edema, and hemorrhage volume were measured at 72 hours after inducing hemorrhage. Inflammatory cells expressing myeloperoxidase (MPO) or OX6 were counted at 48 hours, and western blotting for Akt, pAkt, STAT3, pSTAT3, ERK and pERK was performed at 24 hours. Results : Compared to the vehicle inj
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