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Flow Cytometry and Microscopy as Means of Studying Single Cells: A Short Introductional Overview

Sara Lindström-2012-01-01-Methods in molecular biology
18

TL;DRAbstract

Flow cytometry and microscopy are perhaps the two most commonly used techniques for analyzing single cells. Both techniques are typically robust and provide a high throughput analysis of living and/or fixed cells. The techniques are often combined with fluorescent labeling of cells, using for example antibodies. This chapter is a short introductory review where some of the possible applications using flow cytometry and microscopy are discussed.

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Flow cytometry and microscopy are perhaps the two most commonly used techniques for analyzing single cells. Both techniques are typically robust and provide a high throughput analysis of living and/or fixed cells. The techniques are often combined with fluorescent labeling of cells, using for example antibodies. This chapter is a short introductory review where some of the possible applications using flow cytometry and microscopy are discussed.

Keywords

Flow cytometryFluorescence microscopeMicroscopyCytometryFlow (mathematics)NanotechnologyChemistryCell biology

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