Role of intestinal epithelium in inflammatory bowel disease: effect of cytokines and glucocorticoids on CXCL8 and CXCL10 gene expression and NF-kB signalling in intestinal epithelial cell lines

TL;DRAbstract

Activation of intestinal epithelial cells (IEC) may occur either by luminal antigens stimulation or by inflammatory signals deriving from immune cells in the close vicinity. Production of chemokines by the intestinal epithelium contributes to mucosal infiltration of inflammatory cells in inflammatory bowel disease (IBD). Little is known about the intestinal epithelial expression and secretion of CXCL10 (IP-10) involved in recruiting T-cells and monocytes. Especially, the role of IL-1β, one of the most abundant cytokines in IBD mucosa, has not been established yet. We therefore aimed to evaluate the expression and regulation of CXCL8/Interleukin-8 (IL-8) and CXCL10/Interferon gamma regulated protein 10 kda (IP-10), two of the most abundant chemokines in inflammatory bowel disease (IBD), in epithelial cell lines stimulated with proinflammatory cytokines (IL-1β, IFN γ and TNF-α). NF-κB is known to play a major role in the pathophysiology of IBD. This makes NF-κB a potential phamocological

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