Fluorescent Antibody and Histopathologic Procedures for Hog Cholera Diagnosis
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SUMMARY Hog cholera was diagnosed in 177 herds by examination of specimens from 363 pigs. Specimens from 3,207 pigs in 1,988 herds were examined by fluorescent antibody ( fa ) procedures on tonsil and spleen, and by histopathologic examinations of brain. Both fa frozen tissue section ( fats ) and fa tissue culture ( fatc ) techniques were utilized. The fa tests were positive in 285 (79%), suspect in 41 (11%), and negative in 37 (10%) pigs from infected herds. Encephalitis was detected in 249 (82%) of 304 brains submitted from these herds. There was agreement between tonsil and spleen fats examinations for 361 pigs from 199 herds which had either positive or suspect specimens.
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SUMMARY Hog cholera was diagnosed in 177 herds by examination of specimens from 363 pigs. Specimens from 3,207 pigs in 1,988 herds were examined by fluorescent antibody ( fa ) procedures on tonsil and spleen, and by histopathologic examinations of brain. Both fa frozen tissue section ( fats ) and fa tissue culture ( fatc ) techniques were utilized. The fa tests were positive in 285 (79%), suspect in 41 (11%), and negative in 37 (10%) pigs from infected herds. Encephalitis was detected in 249 (82%) of 304 brains submitted from these herds. There was agreement between tonsil and spleen fats examinations for 361 pigs from 199 herds which had either positive or suspect specimens.
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