Nutrient Regulation and Combined Pretreatment Strategies to Improve the Rate of Delignification by White Rot Fungi

TL;DRAbstract

Delignification is necessary for efficient conversion of lignocellulosic biomass to biofuels. Microbial delignification by white rot fungi is achieved by the action of lignin degrading enzymes, laccase and peroxidase. As the microbial process of delignification is very slow, two strategies have been employed to achieve effective delignification in a short duration. In the first strategy, nutrient regulation of lignin degrading enzymes was exploited to identify the specific carbon and nitrogen sources, their ratio and inducer concentrations and to optimize the nutrients for maximum ligninolytic activity. In the second strategy, fungal delignification was combined with hydrothermal process. A dual effect of copper on Dichomitus squalens laccase activity, inducing and suppressing was revealed. Maximum laccase activity is a function of fungi tolerance to copper and media composition. Some nutrients improved tolerance of cells and laccase-producing system to copper load. Methylated substrat

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