TL;DRAbstract
Raman spectroscopy has been used to investigate different conformational states of bovine pancreatic insulin: the native form\nand several structurally modified states with different extent of denaturation induced by thermo-chemical treatment and by\napplying very high pressure (up to 8 GPa) using a diamond anvil cell. High-pressure results confirm the peculiar strength-tovolume\ncompression of insulin and largely extend the pressure range of its structural stability (0–4.2 GPa). Above 4.2 GPa,\ninsulin undergoes an irreversible structural transition that, once pressure is released, leaves the sample in a new conformational\nstate. The protein secondary structure after the pressure treatment results to be somehow intermediate between the native and\nthe thermo-chemical fibrillar samples. The analysis of the pressure dependence of the Raman spectrum and of several specific\nspectroscopic markers allows us to follow the path from the native to new pressure-denatured protein conformation.
Chat with Paper
AI Agents for this Paper
Raman spectroscopy has been used to investigate different conformational states of bovine pancreatic insulin: the native form\nand several structurally modified states with different extent of denaturation induced by thermo-chemical treatment and by\napplying very high pressure (up to 8 GPa) using a diamond anvil cell. High-pressure results confirm the peculiar strength-tovolume\ncompression of insulin and largely extend the pressure range of its structural stability (0–4.2 GPa). Above 4.2 GPa,\ninsulin undergoes an irreversible structural transition that, once pressure is released, leaves the sample in a new conformational\nstate. The protein secondary structure after the pressure treatment results to be somehow intermediate between the native and\nthe thermo-chemical fibrillar samples. The analysis of the pressure dependence of the Raman spectrum and of several specific\nspectroscopic markers allows us to follow the path from the native to new pressure-denatured protein conformation.
Keywords
Chat
Click to start Chat