Development of stationary phase gradients and multi-functional stationary phases in capillary formats

TL;DRAbstract

The following thesis provides an extensive study into the fabrication of polymer monolithic columns in capillary housing, their modification and subsequent characterisation. The fabricated columns, in all instances, were profiles using scanning capacitively coupled contactless conductivity detection (sC4D), a noninvasive method of characterisation, which can provide information on the density of the monolithic column, and the distribution of charged functional groups within the column.
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\nUsing thermally initiated co-monomer polymerisation, monolithic columns were fabricated within fused silica capillaries. The resulting distribution of charged functional groups was monitored using sC4D. Thermally initiated gradient monolithic columns produced linear gradients, however, the capacity produced was sufficiently low, as determined by the retention factor, k ( k < 1). Alternatively photo-initiated modification of polymer monoliths was investigated using commercially available o

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