Development of a novel enzymatic assay for the detection ofhypoxanthine for freshness estimation

TL;DRAbstract

Hypoxanthine is a naturally occurring purine derivative and one of the substrate for the enzyme xanthine oxides. Xanthine oxidase oxidizes hypoxanthine forming simpler molecule, xanthine. Further oxidation of xanthine by xanthine oxidase produce uric acid and trace amount of hydrogen peroxide as a byproduct. Hypoxanthine derived from common molecule found in muscles (ATP). This paper studies the development of a system to detect hydrogen peroxide in order to establish correlation between the quantity of hydrogen peroxide and the freshness of any A TP containing protein. A system consists of xanthine oxidase, peroxidase and 3,5,3'5'-Tetramethylbenzidine (TMB) is being developed. The effect of hypoxanthine, hydrogen peroxide and 3,5,3' 5' - Tetramethylbenzidine (TMB) concentration were studied via real time scanning using spectrophotometer at 660 run wavelength. Two of these parameters (TMB and hypoxanthine) show direct proportional relation between their respective concentration an

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