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Article10.1111/j.1470-8744.1989.tb00057.x

Free gp70 from FeLV: Enrichment from Cell Culture Fluid by Ferric Oxide‐Agarose Chromatography

Ilya Zelikman,Lennart Åkerblom,Stellan Hjertén,Brør Morein-1989-04-01-Biotechnology and Applied Biochemistry
2

TL;DRAbstract

A new chromatographic material based on beads of macroporous crosslinked agarose containing ferric oxide particles was used for enrichment of gp70--the envelope glycoprotein of feline leukemia virus (FeLV). Free gp70 was purified from cell culture fluid in one step with a recovery of 50 to 60% and a purification of about 60 times. The described procedure is a suitable first step for the purification of gp70 from large volumes of cell culture fluid.

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A new chromatographic material based on beads of macroporous crosslinked agarose containing ferric oxide particles was used for enrichment of gp70--the envelope glycoprotein of feline leukemia virus (FeLV). Free gp70 was purified from cell culture fluid in one step with a recovery of 50 to 60% and a purification of about 60 times. The described procedure is a suitable first step for the purification of gp70 from large volumes of cell culture fluid.

Keywords

AgaroseChromatographyCell cultureGlycoproteinChemistryFerricBiologyBiochemistry

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