Proteomic Analysis of Individual Drosophila Hemolymph

TL;DRAbstract

Developing analysis methods and tools to understand the chemical information of the proteome of an organism of limited volume are challenging and vital for modern proteomic studies. Drosophila melanogaster (also called fruit fly) is one of the ideal animal models for the study of proteomic composition because of the high degree of genome homology with the human genome. Nevertheless, it has less than 50 nL blood (hemolymph) available for collection due to its small size (about 3 mm). The goal of this thesis is to present sample preparation and analysis methods to improve the identification of proteins in volume-limited biological samples using a series of chromatography and mass spectrometry methods. \nA hyphenated nano-reverse phase liquid chromatography chip column-mass spectrometry (nano-RPLC chip column-MS) method was developed to obtain proteomic information of hemolymph from an individual fruit fly. This is the first study to report a qualitative analysis of proteomic composition

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