Cloning and molecular characterization of vffl gene coding forisomes of vicia faba

TL;DRAbstract

Forisomes are phloem-specific proteins of the family of the Fabaceae which undergo a rapid, reversible and ATP-independent comformational change when stimulated by variations in Ca2+ levels or pH. These proteins, present in the sieve elements, switch instantaneously between a dispersed and a condensed conformation, allowing them to control the stream of photo-assimilates in the phloem after wounding by sealing the conducting tubes. Isolation of Forisomes from Vicia faba permitted the sequencing of peptides from which specific oligonucleotides were designed for library screening. In addition, polyclonal Forisome-specific antibodies were obtained. First attempts to identify Forisome-encoding genes were performed by hybridization screening of subtracted and non-subtracted Vicia faba derived cDNA libraries, however, no successful results could be retrieved. In an alternative approach, immunoscreening along with PCR screening of cDNA expression library of Vicia faba plant tips was carried o

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