Cell-to-cell interaction of a mouse dendritic cell and a mouse melanoma cell studied within a microfluidic chip

TL;DRAbstract

Long-term monitoring of biochemical changes on a single cell has provided new information about unusual cellular response to reagents. A microchip device that was fabricated with a cell retention chamber allows selection, retention and shuttling of a single biological cell for long-time analysis. Excellent optical and fluorescent observation of the single cell have been simultaneously achieved. We previously reported this microfluidic method for the analysis on single multi-drug resistance (MDR) leukemia cells because MDR-mediated drug efflux is known to be a major cause of the failure of chemotherapy. This measurement method is dubbed as the same-single-cell analysis (SASCA), which takes advantages of tracking one and the same cell over a long period of time. This method is now employed to study cell-to-cell interaction in cancer research using the mouse cell model. In Australia, melanoma is the most common cancer in the 15-44 year age group and accounts for 3% of all cancer deaths. Q

Chat with Paper

AI Agents for this Paper