T-cell entosis in the liver: catching escaping t-cells and The expression of e3 ubiquitin ligases in t-cells

TL;DRAbstract

Entosis describes a form of emperipolesis, where one cell invades a host cell. Emperipolesis can result in death, division or release of the internalised cell. Preliminary data demonstrates that entosis and release of CD4+T-cells occurs within primary hepatocytes and hepatoma cell lines. 
\nThis study focuses on defining the environment and stimuli necessary to induce T-cell release following entosis. A 7-Day release assay was optimised for measuring CD4+T-cell release following T-cell-hepatoma co-culture and entosis. Flow cytometry was then used to quantify the release of T-cells from HepG2-CD81 and Huh-7 cell lines in response to various stimuli, doses and incubation times. Results indicated that the majority of stimuli and dose responses were not responsible for inducing a higher or lower %T-cell release. Co-cultures also demonstrated a significantly higher %T-cell release from HepG2-CD81s at 2 hour compared to 24 hour incubations in treated and untreated co-cultures. Following

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