A study of the growth of Pseudomonas putida CP1 on mono-chlorophenols

TL;DRAbstract

Pseudomonas putida CPI grew on all three mono-chlorophenol isomers when supplied as the sole source of carbon and energy. The biodegradability of the mono-chlorophenols followed the order: 4-chlorophenol > 2-chlorophenol > 3-chlorophenol. P. putida CPI was able to degrade 300 ppm 4-chlorophenol, 250 ppm 2-chlorophenol and 200 ppm of 3-chlorophenol. In the presence of fructose (1%, w/v) the organism could degrade 400 ppm 4-chlorophenol, 500 ppm 2-chlorophenol and 300 ppm 3-chlorophenol.
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\nChlorophenol removal was stimulated in the presence of low concentrations of glucose (0.05% - 0.5%, w/v). Substrate removal was inhibited and there was a significant fall in pH with concentrations of glucose greater than 1.0% (w/v). When the pH was controlled at pH 7.0 inhibition of substrate removal was alleviated. The rate of removal of mono-chlorophenols was greater in the presence of fructose than in the presence of glucose, yeast extract or a combination of fructose and yeast extr

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