In Situ Hybridization and Immunostaining of Xenopus Brain

doi:https://doi.org/10.1007/978-1-62703-655-9_9

In Situ Hybridization and Immunostaining of Xenopus Brain

Kai-li Liu,Xiumei Wang,Zilong Li,Rongqiao He,Ying Liu-2013-08-27-Methods in molecular biology

3

TL;DRAbstract

The dynamic expression pattern analysis provides the primary information of gene function. Differences of the RNA and/or protein location will provide valuable information for gene expression regulation. Generally, in situ hybridization (ISH) and immunohistochemistry (IHC) are two main techniques to visualize the locations of gene transcripts and protein products in situ, respectively. Here we describe the protocol for the whole brain dissection, the in situ hybridization and immunostaining of the developing Xenopus brain sections. Additionally, we point out the modification of in situ hybridization for microRNA expression detection.

Chat with Paper

AI Agents for this Paper

The dynamic expression pattern analysis provides the primary information of gene function. Differences of the RNA and/or protein location will provide valuable information for gene expression regulation. Generally, in situ hybridization (ISH) and immunohistochemistry (IHC) are two main techniques to visualize the locations of gene transcripts and protein products in situ, respectively. Here we describe the protocol for the whole brain dissection, the in situ hybridization and immunostaining of the developing Xenopus brain sections. Additionally, we point out the modification of in situ hybridization for microRNA expression detection.

Keywords

In situ hybridizationImmunostainingXenopusBiologyGene expressionIn situImmunohistochemistryMolecular biology

Chat

Click to start Chat