Interaction of osmotic and temperature stress on transgenic soybean

TL;DRAbstract

The triphenyttetrazotium chloride (TTC) assay is based on the ability of viable cells to metabolically reduce tetrazolium salts to formazan. It was used as a method to screen the viability differences of soybean plants transformed with a P5CR gene in antisense direction controlled by an inducible heat shock promoter (IHSP). Differences were detected between the transgenics and the control plants using this method. The transgenics exhibited a sensitive reaction in that formazan production was reduced following an osmotic stress treatment. The control plants however exhibited a tolerant reaction in that formazan production was highest during stress treatment. The efficiency of protein synthesis in the transgenic plants was lower than in the control plants when the inducible heat shock promoter was activated. This indicated that underexpression of the P5CR gene resulted in declined protein synthesis due to proline shortage. The transgenics had a lower seed production than the control plan

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