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Open AccessArticle10.1016/s0003-9098(25)00224-3

Genetic analysis of DNA fingerprints for two lines of chickens divergently selected for resistance and susceptibility to a heat stress environment

Essam A. El-Gendy,R.G. Dean,Kenneth Washburn-2000-12-01-Archiv für Geflügelkunde
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TL;DRAbstract

Two lines of chickens derived from a closed base population by divergent selection for resistance (line R) and susceptibility (line S) to a heat stress environment were used to produce DNA fingerprints. Individual DNA samples within line and also mixtures of DNA samples of 5, 10, 15 or 20 individuals per line were cleaved by the Hinf I restriction enzyme and the M13 DNA was used as a probe. The objectives of this study were to estimate the variations within and between lines in DNA fingerprinting patterns, to evaluate associations between these variations and variations between lines due to their performance resulting from selection and to estimate the genetic distance between the two lines. Genetic variability indices of 0.62, and 0.64 were obtained among individuals of lines R and S, respectively. The band sharing (BS) level within line R was significantly higher (0.45) than that of line S (0.34). The average of BS levels between lines was 0.67. The results indicated that line S show

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Two lines of chickens derived from a closed base population by divergent selection for resistance (line R) and susceptibility (line S) to a heat stress environment were used to produce DNA fingerprints. Individual DNA samples within line and also mixtures of DNA samples of 5, 10, 15 or 20 individuals per line were cleaved by the Hinf I restriction enzyme and the M13 DNA was used as a probe. The objectives of this study were to estimate the variations within and between lines in DNA fingerprinting patterns, to evaluate associations between these variations and variations between lines due to their performance resulting from selection and to estimate the genetic distance between the two lines. Genetic variability indices of 0.62, and 0.64 were obtained among individuals of lines R and S, respectively. The band sharing (BS) level within line R was significantly higher (0.45) than that of line S (0.34). The average of BS levels between lines was 0.67. The results indicated that line S show

Keywords

DNA profilingHeat stressGeneticsBiologyResistance (ecology)DNAHeat resistanceComputational biology

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