Fas诱导MML-1凋亡时活化caspase-3表达与细胞周期的关系

TL;DRAbstract

Objective To investigate the relationship between expression of active form of caspase-3 and cell cycle in Fasmediated apoptosis of B lymphocytoma cell line MML-1. Methods MML-1 cells were incubated with agonistic anti-Fas antibody for different time, and cell apoptosis was induced. Cell apoptotic rates were analysed by flow cytometry, and sensitivity of MML-1 cells to apoptosis was determined. The expression of active form of caspase-3 was analysed by double staining with PI-Triton X and FITC-active caspase-3. Cyclin A, B1 and E were selected as cell cycle markers for S, G2/M and G1 phase of MML-1 cells, and the expression of active form of caspase-3 was detected by flow cytometry. Results The cell apoptotic rate reached 56% after induction by Fas for 6 h. After induction by Fas for 4 h, the active form of caspase-3 was mainly expressed in cells of G1 phase, while rarely in cells of S and G2/M phase. Cells with negative cyclin A and B1 and positive cyclin E expressed active form of ca

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