P196-S Novel Software for Automated LC-MALDI and Relative Quantification of Multiplexed iTRAQ-Labeled Samples Using MALDI TOF/TOF Instrumentation

TL;DRAbstract

The new generation of MALDI TOF/TOF instruments can be used to generate informative identification and relative quantification data using iTRAQ chemistries. This presentation introduces a novel automated iTRAQ relative quantification software package for the AXIMA-TOF(2) MALDI TOF/TOF mass spectrometer. The package uses and extends onwards from existing LC-MALDI software. Interfacing of mass spectral data, relative quantification data, and Mascot search results in an interactive, visual correlation providing a highly practical end-user package. The user interface has multi-level organization. The primary level shows Mascot database search hits along with an average quantification ratio for each protein along with significance scoring. The software correlates the peak intensity/area of the iTRAQ reporter ions to the protein identifications assigned by Mascot using MS/MS spectra in order to relatively quantify the protein components in labeled samples. Further interrogation of the indivi

Chat with Paper

AI Agents for this Paper